Identification of chemical components in Zataria multiflora callus by GC-Mass analysis

نویسندگان

  • N. Mosavat* Research Institute for Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan, Iran.
  • P. Golkar Research Institute for Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan, Iran.
چکیده مقاله:

Background and objectives: A modern biotechnological technique to obtain useful natural products from plants is to isolate them from their callus. Zataria multiflora is a bushy herb of the Lamiaceae family known for its stimulant, antibacterial, antioxidant and expectorant effects in Iranian folk medicine.  The present study has investigated the induction of callus tissue and identification of its chemical compounds by GC-Mass analysis. Methods: The plant seeds of were sterilized and cultured in petri dishes lined with MS medium. After the emergence of seedlings, leaf segments were transferred to another MS medium supplemented with 2 mg/L 2,4- Dichlorophenoxyacetic acid + 1 mg/L  Kinetin. The petri dishes were incubated in a growth chamber at 24 °C with photoperiod of 16/8 (light/dark). The methanol extract of the calli were extracted after 2 month of callus induction and the chemicals were analyzed by gas chromatography-mass spectrometry (GC-MS). Reults: GC-MS analysis showed 20 different compound including different fatty acids, phytosterolls and phenolic compounds. Linolenic acid methyl ester (13.38%), thymol (10.34%), cyclohexasiloxane dodecamethyl (7.50%) and р-cymen (7.30%) were the dominant compounds, respectively. Conclusion: This novel   finding showed that in vitro production of thymol and other terpenoids by callus culture could be optimized for wide industrial and pharmaceutical applications via bioreactor systems employment.  

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

In Vitro Anti-Candida Activity of Zataria multiflora Boiss

Zataria multiflora Boiss known as Avishan Shirazi (in Iran) is one of the valuable Iranian medicinal plants. The aim of study was to evaluate anti-Candida activity of Z. multiflora against different species of Candida in vitro. Anti-Candida activity of the aqueous, ethanolic and methanolic maceration extract of the aerial parts of Z. multiflora Boiss was studied in vitro. Anti-Candida activity ...

متن کامل

Chemical composition along with anti-leishmanial and cytotoxic activity of Zataria multiflora.

CONTEXT Natural products and their compounds are some of the most interesting sources of new drugs. Reviews have reported various pharmacological properties such as antimicrobial effects of Zataria multiflora Boiss (Lamiaceae). OBJECTIVE The present study investigates the chemical composition of Z. multiflora essential oil and evaluates its cytotoxic effects and anti-leishmanial activities ag...

متن کامل

Inhibition of Angiogenesis: A Novel Effect of Zataria Multiflora

Background: Angiogenesis, the formation of new blood vessels from preexisting ones, is among the most important physiological and pathological processes that occur in the body. Under pathological conditions such as tumor growth, psoriasis, corneal neovascularization and rheumatoid arthritis, angiogenesis is substantial for the development of the disease. Zataria multiflora is a member of the La...

متن کامل

Identification of volatile oil components from aerial parts of Trigonella torbatjamensis Ranjbar by GC-FID and GC-MS

Objective(s):Various species of genus Trigonella are important from medical and culinary points of view. The essential oil of Trigonella torbatejamensis Ranjbar as an endemic plant in Iran has not been studied previously. The essential oil of this plant was analyzed by different methods for identification of its components. Materials and Methods: The essential oil composition of aerial parts o...

متن کامل

Zatatriol: A New Aromatic Constituent from Zataria multiflora

Muhammad Shaiq Ali*, Muhammad Saleem, and Viqar Uddin Ahmad H. E. J. Research Institute of Chemistry, University of Karachi, Karachi-75270, Pakistan Z. Naturforsch. 54b, 807Ð810 (1999); received July 22, 1998 Lamiaceae, Zataria multiflora, Isopropyl Benzene Constituents, Structure Elucidation, NMR Data A new isopropyl benzene derivative named as zatatriol (5) and two synthetically known compoun...

متن کامل

Benefits of Zataria multiflora Boiss in Experimental Model of Mouse Inflammatory Bowel Disease

Inflammatory bowel disease (IBD) is a chronic condition of the intestine with unknown etiology involving multiple immune, genetic and environmental factors. We were interested to examine the effect of total extract from Zataria multiflora Boiss, a folk medicinal plant on prevention and treatment of experimental IBD. Z. multiflora was administered (400, 600, 900 p.p.m.) through drinking water to...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 4  شماره Supplement

صفحات  46- 46

تاریخ انتشار 2017-11-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023